What is ETS domain?
ETS-domain transcription factors are found in all metazoan organisms and are characterised by the presence of a conserved DNA-binding domain (the ETS-domain). The ETS-domain is also involved in multiple protein-protein interactions in addition to its well defined role in mediating DNA binding.
What is the ETS gene?
In the field of molecular biology, the ETS (E26 transformation-specific or E-twenty-six. (Erythroblast Transformation Specific)) family is one of the largest families of transcription factors and is unique to animals. There are 29 genes in humans, 28 in the mouse, 10 in Caenorhabditis elegans and 9 in Drosophila.
What is ETS fusion?
Fusion of the androgen-regulated gene TMPRSS2 to ERG, which encodes an ETS transcription factor, is found in around half of prostate cancers. The formation of TMPRSS2–ERG results in high level expression of the ERG gene, which is believed to drive cancer development (Figure 1).
What defines a transcription factor?
Transcription factors are proteins involved in the process of converting, or transcribing, DNA into RNA. Transcription factors include a wide number of proteins, excluding RNA polymerase, that initiate and regulate the transcription of genes. Regulation of transcription is the most common form of gene control.
Where are transcription factors synthesized?
the nucleus
In eukaryotes, transcription factors (like most proteins) are transcribed in the nucleus but are then translated in the cell’s cytoplasm. Many proteins that are active in the nucleus contain nuclear localization signals that direct them to the nucleus.
How do you identify transcription factors?
DNA BINDING ASSAYS USED TO STUDY TRANSCRIPTION FACTORS. The principal strategy in identifying and characterizing transcription factors is based on their ability to recognize and interact with specific DNA sequences present in the promoters of eukaryotic genes.
Is p21 a transcription factor?
It has been previously described that p21 functions not only as a CDK inhibitor but also as a transcriptional co-repressor in some systems. We found that p21 rapidly and strongly repressed the mRNA levels of a number of genes involved in cell cycle and mitosis.
